ISMB 2024 | MSAID

Perception and reality of FDR control, data completeness and quantitative precision in (single-cell proteomics) DIA data

Date and Time: Saturday, July 13, 2024 | 11:20 am –11:40 am EDT
Presenter: Martin Frejno

Recently, single-cell proteomics (SCP) moved away from TMT labeling and DDA to label-free experiments and DIA due to its reportedly higher sensitivity and data completeness. Here, we developed a tool that enables routine quality control of peptide-centric DIA results. Application to published single-cell DIA data showed that data completeness was only 45% instead of 98%.
The sky is the limit: a cloud-based proteomics platform for the masses

Date and Time: Saturday, July 13, 2024 | 3:40 pm –4:00 pm EDT
Presenter: Martin Frejno

Laboratories dealing with bottom-up proteomics data often encounter computational hurdles in the journey from raw data to conclusive insights. Challenges arise, from the absence of automated pipelines and disjointed local infrastructure for data storage to processing, systematic result management, and interpretation. The advent of fast-scanning instruments exacerbates these issues by overwhelming local infrastructure with a multitude of files and large raw data sizes. Here, we introduce a highly scalable, fully automatable, cloud-based proteomics platform designed to streamline the entire workflow.

HUPO_2024_MSAID